Metabolism Reactivation in Erythrocytes Cryopreserved with Polyethylene Glycol: Protein State and Reactive Oxygen Species Production
نویسندگان
چکیده
Production of reactive oxygen species (ROS) and protein state membrane-cytoskeleton complex (MCC) in the erythrocytes, subjected to freeze-thawing with polyethylene glycol (PEG) subsequently transferred into physiological conditions vitro after cryoprotective agent removal were examined. Results SDS-PAGE β-mercaptoethanol showed a decrease spectrin content MCC profile cryopreserved erythrocytes. The using diamide revealed an increase accessibility –SH groups for reagent enhanced level high molecular weight polypeptide complexes relative control. A band 8 (peroxiredoxin) erythrocytes by is apparently associated change its localization caused increased ROS production at previous stages cryopreservation. At same time, absence significant changes control upon metabolism reactivation indicated ability cells oxidative processes during short-term incubation.
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ژورنال
عنوان ژورنال: Problems of Cryobiology and Cryomedicine
سال: 2022
ISSN: ['2307-6143', '2518-7376']
DOI: https://doi.org/10.15407/cryo32.02.092